301573 (v.2) Molecular Genetics 334

Area:

School of Biomedical Science

Credits:

25.0

Contact Hours:

8.0

Individual Study:

1 x 5 Hours Weekly

Laboratory:

1 x 3 Hours Weekly

Syllabus:

Principles and applications of molecular genetics. Strategies and approaches used to locate and identify genes involved in conferring a particular trait for example, disease. Chromosome mapping, linkage analysis, gene expression, analysing gene function,gene organisation, immunogenetics and developmental genetics.

Unit Outcomes:

On successful completion of this unit students will be have - Provided an understanding of the methods used to analyse and map genes. Provided an insight into the genetic mechanisms of gene and disease expression. Understood the molecular organisation of gene groups and genomes including comparative genetics. Understood the molecular genetics of development in multicellular organisms. Demonstrated knowledge of basic molecular techniques used for nucleic acid based investigations of genetic diseases/traits. Appreciated and understood the safe use of radioactive substances in a laboratory environment. Performed a range of mathematical calculations related to the manipulation and interpretation of laboratory data. Demonstrated practical expertise with the performance of basic molecular techniques. Demonstrated competence in preparation of reagents for use in nucleic based applications. Demonstrated knowledge of the applications of molecular techniques to understanding the molecular basis of genetic diseases. Understanding of molecular mechanisms involved in cellular processes.

Text and references listed above are for your information only and current as of September 30, 2003. Please check with the unit coordinator for up-to-date information.

Unit References:

Riordan. (1989). Identification of the Cystic Fibrosis Gene - Cloning and Characterisation of Complementary DNA. Science 245, 1066-1072. Rommens. (1989). Identification of the Cystic Fibrosis Gene - Chromosome Walking and Jumping. Science 245, 1059-1065.MacDonald. (1993). A Novel Gene Containing a Trinucleotide Repeat that is Expanded and Unstable on Huntington's Disease Chromosomes. Cell, 72, 971-983. Tilghman. (1999). The Sins of the Fathers and Mothers - Genomic Imprinting in Mammalian Development. Cell, 96, 185-193. Dunlap. (1999). Molecular Basis for Circadian Clocks. Cell, 96, 271-290. Johnson. (1999). Molecular Biology of Aging. Cell, 96, 291-302.

Unit Texts:

Brown, T.A., (1990). Gene Cloning - An Introduction, 2nd ed., Chapman and Hall. Strachan, T. and Read, A.P. (1996)., Human Molecular Genetics, 1st ed. Griffiths, G., Miller and Lewontin. (1999), Modern Genetic Analysis. WH Freeman Publishers.

Unit Assessment Breakdown:

Assignments (2) 30%, Practical test 20%, Examination 50%. This is by grade/mark assessment.

Year	Location	Period	Internal	Area External	Central External
2004	BPI Intern'l Group S'pore	Trimester 1	Y