301251 (v.2) Molecular Genetics 333

Area:

School of Biomedical Science

Credits:

25.0

Contact Hours:

3.0

Lecture:

1 x 3 Hours Weekly

Other Requisite(s):

Admission: upon completion of Diploma of Technology, or equivalent at a Singapore Polytechnic

Syllabus:

Tools of molecular genetics - enzymes, cloning vectors, labelling of DNA. Nucleic acid manipulation techniques - DNA, RNA extraction, hybridisation. Genomic and DNA library construction and screening. Polymerase chain reaction - principle methods, applications in clinical diagnosis. DNA sequencing and applications - Site-directed mutagenesis. Applications of DNA technology - genetic disease and cancer diagnosis. Gene therapy and genetic counselling.

Unit Outcomes:

On successful completion of this unit students will be able to - Demonstrate knowledge of basic molecular techniques used for nucleic acid based investigations. Appreciate and understand the safe use of radioactive substances in a laboratory environment.Perform a range of mathematical calculations related to the manipulation and interpretation of laboratory data. Demonstrate practical expertise with the performance of molecular techniques. Demonstrate competence in preparation of reagents for use in nucleic based applications. Demonstrate knowledge of the applications of the molecular techniques.

Text and references listed above are for your information only and current as of September 30, 2003. Please check with the unit coordinator for up-to-date information.

Unit References:

Brown T.A., (1992), Genetics - A Molecular Approach, 2nd ed., Chapman and Hall. Mathews and Van Holde, (1990), Biochemistry, Benjamin Cummings Publishing Company. Lewin B., (1994), Genes V, Oxford University Press Inc. Wills C., (1992), Talking Genes, The Science Behind the Human Genome Project, Oxford University Press.

Unit Texts:

Brown T.A., (1998), Gene Cloning - An Introduction, 3rd ed., Stanley Thomes Ltd. Old Primrose (Ed.), (1994), Principles of Gene Manipulation, 5th ed., Blackwell Scientific.

Unit Assessment Breakdown:

Mid Semester Examination 20%. Practical Tests and Assignments 50%. Final Examination 30%. This is by grade/mark assessment.

Year	Location	Period	Internal	Area External	Central External
2004	BPI Intern'l Group S'pore	Trimester 1	Y
2004	BPI Intern'l Group S'pore	Trimester 2	Y